Heparome is a database which contains the information of heparin-binding proteins of E. coli K-12 MG1655 cells. Heparin affinity columns were applied to enrich and fractionate proteins. Identification of proteins was done via the collaboration with David Russell's lab.
Because heparin is negatively charged sulfated glucosaminoglycan, polyamion binding proteins, which contain nucleic acid-binding proteins, are expected to bind to heparin columns. Study of the expression pattern of heparin-binding proteins will help to study the nucleic acid-binding proteins, most of which are related to regulation. Moreover, heparin affinity columns will also erich low abundance proteins.
Heparome database is constructed using MySQL. Website interface is built using HTML and PHP. Queries between MySQL database and website interface are executed using PHP. Besides including information of identified proteins, such as swiss accession number, gene name, molecular weight, isoelectric point, condon adaptation index (CAI), functional classification, et. al. , it also includes information of experiments, such as sample preparation, heparin-HPLC chromatography, SDS-PAGE gel separation and MALDI-MS.
Last modified: May 17 2006 17:25:33.
